208 LILY H. HUIE. 



vacuoles appear to be filled with a substance which stains 

 pale blue. In a few tentacles there is a reddish tinge in the 

 apical half of the cells. The nucleus is normal as to position, 

 size, and shape. The nuclear plasm stains occasionally bluer 

 than in controls (fig. 4) ; or again in those cells which show 

 a tendency in the cell plasm to stain red, the nuclear plasm 

 takes on the same tint. There is, therefore, at this stage a 

 decrease in the basophile affinity which was so marked a 

 characteristic of the earlier stages. The nuclear plasm is not 

 diminished in quantity or altered in arrangement. 



The chromosomes are not perceptibly increased in size, but 

 appear to be slightly more numerous. In some nuclei they 

 appear as if united by fine threads of a substance which also 

 stains dark blue. The nucleolus is normal. 



The nuclei of the third layer are either normal or very 

 slightly shortened. 



One Hour after Feeding (figs. 6 — 8). — The droplets 

 adhering to the exterior of the tentacle and those on the 

 lateral walls of the gland cells generally stain more faintly 

 and are less conspicuous than in earlier stages. 



The cell walls stain extremely pale blue. 



The cytoplasm is paler blue and less dense than, in earlier 

 stages, but is quite as full or fuller than in most controls, and 

 its mass is often outlined with dark drops or granules. 



The nucleus is sometimes normal in size and shape, and 

 sometimes shrunken. The nuclear membrane has quite dis- 

 appeared. The nuclear plasm stains like that of controls, and 

 in those nuclei which have not shrunken it is arranged as in 

 controls, while in the shrunken nuclei its arrangement cannot 

 be made out. 



The chromosomes have increased enormously. In those 

 nnclei which have not shrunken they are seen to be ribbon- 

 shaped. I have not found it possible to determine whether or 

 not they exist in any fixed number. 



The nucleolus is smaller than normal. In the unshrunken 

 nuclei it is surrounded by a clear zone. 



In the third layer the precipitate is as heavy as before. 



