CYTOLOGIOAL CHANGES PRODUCED IN DROSERA. 209 



a 



The nuclei vary in shape from being only slightly shorter than 

 norroal to forms such as are shown in figs. 32 and 33 of my 

 previous paper. 



One Day after Feeding (fig. 9). — After the leaves had 

 been fed it was unfortunately necessary to remove the plants 

 from their natural habitat. They were placed in a cool 

 greenhouse, where the leaves that were allowed to remain 

 growing reopened healthily in two to four days, but it is 

 possible that the transplanting may have aflfected the cyto- 

 logical appearances about to be described. It is remarkable 

 that there was nothing to be seen resembling the precipitates 

 of the earlier stages either externally or internally. 



The cell walls are very pale blue, almost colourless. 



The cytoplasm is purple, very granular, greatly vacuolated 

 iu the apical half of the cells, dense in the basal half, though 

 showing here also small vacuoles. In the neighbourhood of 

 the large vacuoles dark blue granular matter collects. 



The nucleus is normal in size and shape, and is surrounded 

 by a membrane, which facts suggest that recuperation has 

 begun in the nucleus, though not as yet in the cytoplasm. 



The nuclear plasm is of the same tint as the cytoplasm, and 

 is irregularly disposed, instead of being regularly distributed 

 as seen in controls and in the earliest stages of peptone 

 feeding. 



The chromosomes are large and angular, drawn out into 

 points, which frequently gives them a stellate appearance. I 

 have not ascertained whether their number is constant. 



The nucleolus is not very much smaller than normal. 



The nuclei of the third layer are globular or irregular in 

 shape, pale pink, nearly destitute of nuclear plasm, with a 

 few conspicuous chromatin granules and a diminished nu- 

 cleolus. They resemble fig. 32 of my former paper. 



Ammonium Sulphate. — As Kiihne's amphopeptoue is 

 prepared with ammonium sulphate, it was thought advisable to 

 ascertain whether the precipitates formed in the cells by feed- 

 ing with amphopeptone might be due to traces of this salt. 

 Ammonium sulphate in 10 per cent., 100 per cent., and 200 



