210 LILY H. HUIE. 



per cent, solutions in distilled water was therefore applied to 

 the leaves. No precipitates resulted, and vacuolation of the 

 cytoplasm was the only eflFect produced in the cells. 



Some experiments were also tried with Witte's peptone, 

 which is very rich in albumoses. The tentacles did not close 

 rapidly, but in from one to four hours they had all closed. 

 The bending caused was more rapid than that induced by the 

 fibrin peptone. In about three days the leaves reopened. 

 The material fed with Witte's peptone was accidentally 

 spoiled and rendered useless for histological examination. 



Before describing, however, the changes induced by fibrin- 

 peptone it is best, for comparison, to detail alterations caused 

 in the gland cells by Griibler's fibrin. 



Fibrin (stains Red). — Maximum change shown in fig. 11. 

 The fibrin was reduced to a powder by crushing, and was then 

 applied either dry or after being moistened with distilled water. 

 If all the tentacles were loaded they closed in about ten 

 minutes, otherwise only those that were touched closed, and 

 did so more slowly. The leaf itself did not double up as after 

 feeding with white of egg. Cytological changes : 



Five Minutes after Feeding. — The only observable 

 changes are that the cytoplasm is vacuolated in the upper half 

 of the cell, and the nucleolus somewhat diminished. 



After One Hour (fig. 10 a). — Tiie cell wall is paler blue 

 than normal. The cytoplasm pale blue, much vacuolated ; fre- 

 quently the cell is almost emptied. The nucleoplasm is aggre- 

 gated. The chromatin is normal. The nucleolus has dimin- 

 ished to about one half its original diameter. 



After One Day (fig. 10 b). — The cell wall is almost colour- 

 less. The cytoplasm is reduced to a scanty pale blue reticulum. 

 The nucleoplasm is normal as to colour, or redder, aggregated 

 and diminished in amount, showing empty spaces. The chro- 

 mosomes have enlarged. The nucleolus is always diminished, 

 sometimes reduced to a mere point. 



After Two Days (maximum change, fig. 11). — The cell 

 walls are colourless or slightly pink. The cell plasm is blue, 

 extremely scanty. The nucleoplasm is thin, pale purple. The 



