212 LILY H. HUIE. 



dish and becoming more scanty. The chromosomes are normal. 

 The nucleolus is diminished. 



One to Two Days after Feeding (usual type, fig. 14). — 

 The cell walls are very pale blue. The cytoplasm is extremely 

 scanty, and stains very pale blue. The nuclear membrane is 

 indistinct. The nuclear plasm is reddish and scanty. The 

 chromosomes have slightly enlarged. The description just 

 given is typical for the state of " maximum change '"' usually 

 produced by milk in one to two days. It will be seen that the 

 nuclear changes, especially those affecting the chromatin, are 

 comparatively small. I have found this to be characteristic of 

 milk. In a few cases, however, the nuclear changes have pre- 

 sented the appearance shown in fig. 15, which must be regarded 

 as exceptional. The nuclear membrane is scarcely traceable. 

 The nuclear chromosomes are much enlarged, angular, and 

 very clear. They closely resemble those produced by calcium 

 phosphate feeding (see fig. 16). The nuclear plasm has almost 

 entirely disappeared. The nucleolus is pale red and very 

 small. Recuperation proceeds in the usual way, and is very 

 complete. 



Calcium Phosphate (fig. 16), — The salt did not adhere 

 to the tentacles after fixation, and so does not appear in stained 

 material. When treated with a mixture of the two stains 

 employed it seems to take up both, assuming a violet tint. 

 The salt was applied as a dry powder. The leaves closed fairly 

 rapidly. In two to three days they reopened, showing still 

 the white powder apparently unaffected. 



Changes induced in Five Minutes. — The cell wall 

 stains a deeper blue than normal. The cell plasm shows a 

 slightly increased vacuolation. The nucleus is contracted to 

 about two tiiirds of its normal size. The nuclear plasm stains 

 deep blue. The chromosomes and nucleolus are normal. 



One Hour after Feeding. — The cell wall is blue. The 

 cell plasm shows increased vacuolation. The nucleus is con- 

 tracted. The chromosomes are normal. The nucleolus is 

 diminished somewhat. 



One to Two Days after Feeding (maximum change, 



