CYTOLOGIOAL CHANGES PKODUCED IN DROSERA. 213 



fig. 16).— The cell walls are pale blue. The cytoplasm is 

 exhausted and very pale blue. The nucleus is contracted 

 irregularly. The nuclear plasm is scanty — very pale violet or 

 blue. The chromosomes are greatly enlarged. The nucleolus 

 is diminished^ sometimes excessively small, and stains dull red. 



The maximum changes thus described closely resemble con- 

 ditions produced by milk (fig. 16). 



In Two to Three Days. — Recuperation occurs. The 

 cytoplasm remains rather poor. The nucleus becomes quite 

 normal. 



Globulin (stains Red) (maximum change, fig. 17). — The 

 leaves were dusted with the dry powder and closed rapidly, 

 i. e. seven to ten minutes. The globulin was entirely absorbed, 

 and the leaves reopened in about three days quite clean. 

 Some of them were fed a second time. 



Cytological Changes: Five Minutes after Feeding. 

 — The cell walls stain very pale blue or purple. The cyto- 

 plasm is diminished in amount, and large vacuoles appear in it. 

 It stains blue or slightly purple. The nuclear plasm shows 

 slight aggregations. The chromosomes and nucleolus are 

 normal. 



One Hour after Feeding. — The cell walls do not stain 

 at all. The cytoplasm is grey and scanty. The nucleus loses 

 its sharply defined contour, and its outline is only indicated 

 by the dark blue circle of the somewhat enlarged chromo- 

 somes. The nuclear plasm is aggregated, and has become more 

 eosinophilous. The nucleolus has diminished, and stains less 

 brightly. 



One Day after Feeding (maximum change, fig. 17). — 

 The food in immediate contact with the apex of the tentacle 

 appears at this stage striated with colourless lines issuing in a 

 radiate manner from the apical cells, and suggesting that 

 secretory products pass out through the apical walls. At the 

 same time the food seems to be passing in between the lateral 

 walls of the cells, which seem to stand apart, apparently sepa- 

 rated by the red-stained globulin. The cell walls stain red. 

 The cytoplasm is pale blue, or grey and scanty. The nuclear 



