1914) Smith—The Development of Anopheles punctipennis Say 2 
spent its whole life in the cage. In the spring and summer they 
die within three days, under the same treatment of water and 
food. 
A larva of A. punctipennis was frozen solid on Decemker 3, was 
thawed out on December 4, and in an hour was apparently normal. 
The full-grown larve found in the autumn differed more from 
one another than those found in the spring and summer, when they 
are mostly green or brown. In autumn there were many more of 
the striped and speckled forms. They all developed into Ano- 
pheles punctipennis, with some differences in size. 
Pool 2 did not yield larve of A. punctipennis in the spring, 
having dried up completely, and pool 1 was much later in devel- 
oping them than 3 which was near by—within a hundred feet. 
Pool 3 on May 24 yielded larvee of all sizes. Most of them, 
however, were ready to pupate. They represented the first laying 
of the spring. The water of this pool was several feet deep at one 
end, and clear and cool. No larve have been found at this spot, 
in the shade of an overhanging bank where Spirogyra grows 
deep, giving its clear green hue from below the surface. A 
few feet away, in shallow water with Spirogyra protruding from 
the surface, were plenty of larvee. With the Spirogyra, and also 
in fruiting condition, was plenty of Zygnema. In and above these 
mats of fruiting alge, many of them feeding down among the fila- 
ments, were larvee of A. punctipennis, nostly full grown. And with 
them in great numbers were several species of Chironomid larve, 
weaving their houses of the delicate threads, and feeding there as 
well. This pool was visited at intervals, until on June 10 there 
were scarcely any larve, but a few pupexe and plenty of empty 
pupa cases. On June 17, however, great numbers of very small 
ones from 1 to 2 mm. and less were to be found again in pools 3 
and 1. In 1 they were found only among the Lemna, and in 3 
only in mats of Spirogyra and Zygnema. Meanwhile, multitudes 
of toad tadpoles had developed in pool 3, and along with their 
growth went the disappearance of the mats of alge from the sur- 
face, and also of Anopheles punctipennis from that part of the 
pool. They were found a month later in almost the same place in 
great numbers among fruiting Chara, which had spread from the 
deeper part of the pool. There was protection and food among 
its filaments. 
