44 CASPER 0. MILLER. 



veloped from a single zoospore or by the fusion of a number 

 of zoospores. 



Cienkowski (6 and 7) planted spores of Licea pannorura, 

 Wallr., on decomposing carrots, and obtained plasmodia. 

 He also planted the spores in water placed on slides, and saw 

 the zoospores fuse to form plasmodia. Spores of Physarum 

 album = Chondrioderma difforme, planted on microscopic- 

 ally small portions of vegetable fibre, developed plasmodia on 

 the fourth day, and twenty-four hours later they fructified, 

 so that under good conditions they completed tiieir cycle of 

 development in five days. 



Lieberkiihn (9) described a plasmodium which he found in 

 the bottom of a glass vessel in which spougillia were being 

 cultivated. 



Cienkowski (16) cultivated Didymium libertianum in 

 water. In one to two weeks plasmodia appeared in the water 

 or creeping on the wall of the vessel. 



He also found a plasmodium in fresh water containing algae. 

 He studied it in hanging drop-cultures and on the slide. He 

 thought it probably was the same species which Lieberkiihn 

 had studied. Sporangia did not form in any of his cultures. 



Stahl (22) cultivated ^Ethalium septicum on moist tan, 

 and saw a species of Physarum form small-stalked sporangia 

 on a filter- paper culture. He did not use any aseptic pre- 

 cautions, and does not state how long it took the sporangia to 

 form after planting the spores. 



Ward (25) found a plasmodium which formed sporangia on 

 the roots of hyacinths which he was cultivating in water con- 

 taiuing a small percentage of salts of lime, magnesia, potash, 

 and soda. He then made a decoction of hyacinth roots, which 

 he boiled and used to make drop-cultures. By planting the 

 spores he succeeded in getting the zoospores and plasmodia in 

 drop-cultures and on slides without other forms than bacteria. 

 The cover- glasses were heated, and the cardboard used in 

 making the moist chambers was boiled. 



Strasburger (26) obtained Chondrioderma diff. by 

 placing macerated stalks of Vicia faba on moistened filter- 



