THE ASEPTIC CULTIVATJON OP MYOETOZOA. 45 



paper under a bell-jar ; the sporangia developed after a few days. 

 He also made drop-cultures of the spores of Chond. diff. 

 in a decoction of cabbage-leaves or bean-stalks_, leaving frag- 

 ments of vegetable fibre in the fluid. He heated the cover- 

 glasses and needle used in making the inoculations^ but added 

 algae and bacteria to the cultures. In many of the cultures 

 the development did not go further than the formation of 

 microcystSj but in more favorable cultures plasraodia de- 

 veloped which fused with each other, and on the fourth or 

 fifth day tliey crawled out from beneath the cover-glass and 

 formed sporangia. 



Wingate (32), in describing Enteridium rozeanum, 

 says that Roze (12) cultivated plasmodia in earthenware dishes 

 filled with sphagnum and water, into which he thrust dead 

 branches of trees, pieces of decayed stumps, &c., which were 

 taken from the neighbourhood of Paris to America. He ob- 

 tained various plasmodia, and studied them until they formed 

 sporangia. I have unfortunately nut been able to procure the 

 original work by Roze. 



Lister (34) cultivated Chond. diff., and obtained the 

 sporangia in from ten to fourteen days after planting the 

 spores. Tiie writer has only seen a short report of the paper 

 in Just's ' Jahresbericht,' so that he does not know what 

 methods were employed. 



Celakovski (38) used the method which Pfefi'er (35) found 

 useful for obtaining plasmodia. He placed dried stalks of 

 Vicia faba, or the leaves and stalks of other plants, par- 

 ticularly of Typha latiflora, in broad crystallising dishes, 

 poured enough water in the dishes to cover the greater portion 

 of the nutrient material, covering the dishes with suitable lids, 

 and sterilised them at a boiling temperature. He then planted 

 spores of Chondrioderma diff. and Didymium macro- 

 carp on. In from six to fourteen days plasmodia of the former 

 were found in the cultures. He frequently obtained the two 

 plasmodia together by simply moistening the stalks of Vicia 

 faba, and placing them in a covered dish. By repeatedly 

 transplanting he obtained the Didymium alone, without the 



