54 CASPER 0. MILLER. 



membranous expansions vary a good deal in sporangia from 

 the same culture. The spores are of a light violet colour ; they 

 have a smooth membrane, and are tolerably uniform in diameter, 

 measuring 7"9— 8'5/x. 



The sporangia of Stemonitis C resemble those of Stemo- 

 nitis B. The sporangia of Stemonitis C measure 3*3 — 

 3*7 mm. in length, and 0*37 mm. in thickness. The columella 

 is not infrequently bent on itself at about the upper four fifths. 

 The secondary fibres of the capillitium are longer than in the 

 sporangia of Stem. B. The stalk measures 0'68 — 1'16 mm. 

 in length. The spores are smooth, of a brownish- violet colour, 

 measuring 7*4 — 11 fx in diameter. 



It would therefore appear that the differences between the 

 sporangia of Stemonitis B and C are not less than those 

 which separate some of the forms which are described in 

 works on the subject under different names. It is possible 

 that further cultivation may show that they are the same. 



Hay and Leaf Cultures. 



In cultures made with unsterilised hay in jars without 

 aseptic precautious, or in flasks with aseptic precautious, 

 one finds bacteria, fungi, monadina, infusoria, and plasmodia 

 developing with uniform regularity. Chondrioderma dif- 

 forme and some species of Didymium, usually micro- 

 carpon, appear togetherorsingly, the Chondrioderma being 

 most frequently present. As has been stated before, some Plas- 

 modium appears in every culture made with unsterilised hay. 



By the drying method the Chondrioderma diff. and 

 Didymium microcarpon have been separated and culti- 

 vated aseptically in flasks. They both form sporangia on the 

 hay, and on the glass above the hay. 



In a culture of Chond. diff. made in dilute hay infusion 

 with 2 per cent, milk added, which had been kept in the dark 

 for several weeks and then placed in the light, sporangia 

 formed under the surface of the water. The sporangia were 

 small, round, or pear-shaped, and did not show the presence of 



