HEART-BODY, ETC., OP CERTAIN POLYCH^TA. 297 



make out the nucleus in them ; but in some pale yellow cells, 

 occurring just posterior to the stomach, it can be seen. That 

 it was the nucleus that was stained in these cells was shown by 

 the action of acetic acid, which revealed no other nuclei. Dr. 

 Mayer, to whom I showed a preparation, described the appear- 

 ance as a faint but distinct nuclear stain, though he was 

 inclined to regard the carmine as in the form of granules in 

 the nuclei. 



It should be stated that carmine partially dissolves in sea- 

 water, and this solution will stain fixed tissues ; and also that 

 the contents of the gut of Siphonostoma are faintly alkaline. 



Bibliography. 



1. Allen. — 'Quart. Journ. Micr. Sci.,' vol. xxxiv, (Gill excretion in Crus- 



tacea.) 



2. Ambkonn.— ' Mitt. Zool. Stat. Neap.,' vol. ix, 1890, p. 475. (Cellulose 



reaction in chitin.) 



3. Barfuth.— ' Arch, fur mik. Anat.,' Bd. xxv, 1885, p. 269. 



4. Beddard. — 'Monograph on Oligochseta' (Clarendon Press), p. 77. 



(" Heart- body " in Oligochseta.) 



5. Bles. — ' Report of Brit. Assoc.,' 1891, p. 373. (Siphonostoma.) 



6. Buchanan (Miss).— (A) ' Quart. Journ. Micr. Sci.,' xxxi. (Hekatero- 



branchus.) 



7. Buchanan (Miss).— (B) ' Report of Brit. Assoc.,' 1895, p. 469. (De- 



velopment of Magelona.) 



8. Buchanan (Miss).— (C) 'Quart. Journ. Micr. Sci.,' xxxv. (Polynoid 



with branchiae.) 



9. Claparede. — (A) ' Annelides Clietopodes du Golfe de Naples,' Geneva, 



1868. 

 9'. Claparede. — (B) ' Rech. Annelides Sed.,' p. 94. 

 9". CuENOT.— ' Arch, de Zool. Expt.,' 2« ser., torn, ix, p. 438. 



10. Cunningham.—' Quart. Journ. Micr. Sci.,' xxviii, 1888, p. 259. (Heart- 



body.) 



11. Daddi.— ' Arch. Ital. Biol.,' xxvi, 1896, p. 143. (Fat test.) 



