376 R. R. BENSLEY. 



cells do secrete small quantities of pepsiu^ but a study of the 

 positive characters of the cell reveals that they possess different 

 staining characters from the chief cells lower down, and are 

 engaged in a different kind of secretion. 



I shall describe in the first place the cells from the upper 

 portion of the gland neck (fig. 6, a). These are conical or 

 pyramidal in shape, and wedged in between the large ovoidal 

 border cells of this portion of the gland in such a way that in 

 vertical sections their broad end is usually directed towards 

 the lumen. In sections stained in hsematoxylin and eosin the 

 cell is divided into two zones, an outer protoplasmic zone 

 staining readily in eosin, and an inner zone engaged in secre- 

 tion which stains with difficulty. The outer zone consists of 

 two elements, fine fibrillse which join one another to form a 

 network, and a hyaline substance filling up the interstices of 

 this network. The inner zone exhibits a structure similar to 

 that of the chief cells of the body of the gland, and for similar 

 reasons the accumulation of droplets of secretion forces the 

 protoplasm to simulate the appearance of a reticulum, although 

 the bars which compose it are not nearly so thick nor so 

 regularly arranged as in the chief cells lower down. 



The secretion in the cells of the gland neck stains intensely 

 in Bordeaux 11 and indulin, and these dyes, particularly the 

 latter, have rendered me considerable service in determining 

 the distribution of this kind of secretion in the stomach, and 

 also in studying the secretive processes in the cells containing 

 it. 1 have found the most convenient method of applying this 

 dye to be in the form of Huberts blood-staining fluid, con- 

 sisting of two grammes each of aurantia, eosin, and indulin, 

 rubbed up in a mortar with thirty grammes of pure glycerine. 

 This fluid is diluted with from 100 to 400 times its volume of 

 distilled water before use, and allowed to act on the sections 

 transferred to it from water for five to thirty minutes. The 

 sections are then washed in water, dehydrated, cleared in 

 benzole, and mounted. On examination it is found that the 

 red blood-corpuscles are stained yellow, the nuclei of all cells 

 a faint hsematoxylin tint, the border cells and chief cells of 



