62 



up, each containing a definite amount of the extract, 

 together with the substance to be acted upon. The 

 " liver " extract was neutral, perhaps, if anything, slightly 

 acid in reaction to litmus. The following were the 

 various experiments made: — 



(1) 5cc. ^ HCl + 4cc. dist. H.O + 3cc. extr. + -dgv. fibrin. 



(2) 5cc.^ NaoCOg + 4cc. dist. H^O + 3cc. extr. + -dgv. fibrin. 



(3) 9cc. dist. HoO + 3cc. extract + •5gr. fibrin. 



N 



(4) Sec— j-TT NaaCOs + 4cc. dist. H.^0 + 3cc. extr., allowed to 



stand three hours, and made acid. 



N 



(5) 5cc. -ypT-HCl + 4cc. 2 % peptone + 4 cc. extract. 



N 



(6) occ -YpT Na.^COa + 4cc. 2 % peptone + 4cc. extract. 



(7) Sec- HoO + 4cc. 2% peptone + 4cc. extract. 



(8) 5cc. 1% Methyl Acetate Sol. + 2cc. extract. 



(9) 5cc. 1% Methyl Acetate Sol. + dist. HgO (as control). 



To each of these two drops of HCN were added as a 

 preservative. 



(10) Extract + Starch Solution ; toluol as preservative. 



After allowing the various mixtures to stand for 

 forty-eight hours, the following tests were made: — 



The residues from 1, 2, -j, 4 and 7 were divided into 

 three parts respectively, and the following products of 

 digestion sought for — peptone or albnmoses, ty rosin, and 

 tryptophane. 



The Biuret test consists in the addition of one drop 

 of CuSOj sol. and then KHO — a rose coloration indicates 

 albumoses, a violet colour indicates unchanged protein. 



Tyrosin is tested for by adding Millon's reagent to a 



