91 NOTliS AND MEMOKANUA, 



larger, in order to deprive them of the calcareous coating, 

 and by the use of staining media, that Professor Schulze 

 succeeded in demonstrating the existence of the nucleus. 



In Entos'olenia glohosa (Williamson) the author found 

 a combination of several conditions which rendered it a 

 favourable object for study in this regard. Its ovate or 

 elongate calcareous shell, deeply invaginated at one end, is 

 hyaline, delicate, and smooth, and perforated by extremely 

 fine pores; the body-mass usually quite or almost quite fills 

 the cavity of the shell, though it can become reduced to a 

 ball lying at the " posterior " eAd of the shell, such seem- 

 ingly due to diminished nutriment. In the finely granular 

 sarcode body-mass there occur embedded numerous strongly 

 refractive, probably oleaginous, partly culourless, partly 

 brownish roundish corpuscles, amongst which, towards the 

 posterior end, the author could see even in the living ex- 

 ample a distinctly marked, clear, globular body. That this 

 was a corpuscle and not a vacuole or a granuleless spot in 

 the sarcode was concluded from the constancy of its ])Osi- 

 tion and occurrence, as well as from the sharpness of its 

 contour. Upon application during observation of dilute 

 acetic or pyroligneous acid, the pores in the shell be- 

 came at first more evident, then the shell became dissolved 

 almost completely, and the grannies embedded in the sar- 

 code became so nearly deprived of all colour, tliat this now 

 finely granular body stood out distinctly, as against its pale 

 environment, and Avith a sharply marked contour, as an 

 indubitable cell-nucleus. 



But in the many-chambered calcareous Foraminifera the 

 demonstration of the nucleus was accompanied by greater 

 difficulty. 



For several reasons the author chose Polystomella striato- 

 punctata as the starting-point of the research : first, the 

 readiness with which he could obtain it in different condi- 

 tions ; secondly, its regular structure Avas in favour of its 

 study ; thirdly, its shell was of comparatively small mass, 

 and less troublesome to eliminate by means of acids ; and 

 lastly, he wished to study this form owing to its having 

 been so largely the subject of research by preceding ob- 

 servers. 



The arrangement and figure of the successive "chambers," 

 and the finger-like conjoining processes are best seen in the 

 sarcode-mass after removal of the calcareous shell. Athough 

 Max Schultze first experimented in this way he did not 

 appear to have detected the mutual bridge-like connection 

 of the body-segments belonging to each chamber, but, on 



