274 D. D. CUNNINGHAM, 
sporange was always highly developed. In some cases curious 
particoloured or piebald sporangia were present in which local- 
ised portions of the contents consisted respectively of cellules and 
of normal spores. The size of the cellules varied considerably 
in different instances, ranging from that previously given down- 
wards to specimens measuring ouly 3 or 4X2°7u; on other 
occasions in which there was no microscopic evidence of their 
presence, isolated masses of them were encountered among the 
Amcebe and sporoids within normal sporangia or in those in 
which only an imperfect spore-formation had taken place. 
When the masses of cellules are allowed to remain in a suit- 
able nutritive fluid, the gelatinous investing substance in which 
they are embedded gradually dissolves and disappears leaving 
the individual cells free. These now show a slight gradual 
increase in size, the oily matter of the nucleus gradually dis- 
appears leaving the greenish nucleolar particle very conspicuous, 
and a minute contractile vesicle generally appears. The outline 
of the cell also becomes somewhat modified, for while one 
extremity retains its original pointed character, the other be- 
comes somewhat rounded. Subsequently one or more flagellar 
filaments are protruded from the latter, and the body swims off 
as an active pear-shaped nucleated zoospore of minute size. 
The zoospores after continuing their active movements for some 
time, and in doing so frequently exhibiting very extensive ame- 
boid changes of form, gradually cease to move, becoming at the 
same time more or less rounded, and finally creep off as minute 
Ameebee (PI. XVIII, fig. 16 a, 4, ¢, e, 7). Both zoospores and 
Amcebe generally show the nucleolar particle originally present 
very distinctly. In other cases the flagellated zoosporic stage 
seems to be omitted and the cells, after undergoing a certain 
amount of increase in size, pass off at once as nucleated Amebe. 
In one or two instances I have met with large active Ameebze 
containing varying numbers of these cells within them, but 
whether this were a case of ingestion or of commencing develop- 
ment, could not be ascertained (Fig. 21). These cells certainly 
Fic, 21.—Large Amcba containing a mass of fusiform spores x 1000. 
