MICROSCOPIC ORGANISMS IN INTESTINAL CANAL. 277 
addition to the ordinary scattered granules, contained a more or 
less altered red blood-corpuscle in their interior. An abun- 
dance of still molecular matter was also present in the serum, 
but no active bacteria could be detected. On the next day the 
serum was full of circular cells, either motionless or still ex- 
hibiting slow form-change, while a few continued to progress 
slowly. The average diameter of the circular cells was about 15 pu, 
some of them contained three nucleoli. ‘They appeared as bright, 
white, shining bodies in the yellow-stained serum. ‘Their pro- 
toplasmic contents in some cases were aggregated into a central 
or lateral granular mass, leaving the rest of the body apparently 
occupied by a homogeneous fluid; a few much larger bodies 
were present, attaining in some cases a diameter of 45, and of 
an even molecular substance. The still, amcebal bodies com- 
pletely filled the fieldin many parts of the preparation, especially 
an the margins of the clot, were they formed continuous sheets 
and masses. A fresh drop of blood was now taken, inoculated 
from the previous one and sealed like it ina wax-cell. The initial 
phenomena in this were just those characteristic of normal blood. 
‘Twenty-four hours later, however, the clot had become of a dark 
red-brown colour, and the serum contained much molecular 
matter and numerous slowly moving amceboid bodies. On the 
subsequent day, the latter were again observed, the activity of 
movement being now more decided. ‘They contained a dim 
nucleolus within a clear nuclear area, but were devoid of any 
contractile vesicle. Twenty-four hours later the serum was full 
of small Amcebe, measuring ahout 10m in diameter when cir- 
cular, and provided with a distinct nucleolus. After another 
interval of twenty-four hours the clot was found to be almost 
everywhere surrounded by a bank of circular and slowly moving 
binucleolate Amcebe, measuriag when at rest about l5y in 
diameter, and appearing as bright punched-out areas in the 
brownish serum. 
A second transfer was now made as before, inoculation being 
effected by means of a needle which had, as formerly, been 
heated to redness, and allowed to cool immediately previous to 
the operation. In this case the results were of a similar nature 
to those in the previous experiment; only the appearance of the 
Ameebe was somewhat retarded. Ultimately an enormous accu- 
mulation of still amceboid cells was formed as before. A third 
transfer was next carried out, but was followed by no develop- 
ment of Amcebe, the basis merely rapidly breaking up and be- 
coming full of molecular matter. The experiments as they stand, 
however, clearly show that the spores of the organism, although 
finding their natural medium in excretal matters, are perfectly 
capable of life and activity in other media. In some of the 
