MICROSCOPIC ORGANISMS IN INTESTINAL CANAL. 279 
hours it had been replaced by strong alkalinity. ‘The surface of 
the material was now covered with a creamy greyish-yellow layer 
of bacteria, which at once began to move actively in nutritive 
fluid. On the following day it was inoculated with one or two 
normal sporangia from a dried cultivation of cow dung, the 
condition of the sporangia being specially favorable to their ready 
transfer. Two days later the cultivation was again examined. 
The basis retained its highly alkaline reaction, but the bacterial 
rods had now been almost entirely reduced to series of spores 
(Fig. 3), so that the surface coating consisted of little save dense 
masses of brightly refractive granules. Inthe gelatinous matter 
of this coating numerous large Amebe were slowly crawling 
about. None of them at this time showed a contractile vesicle, 
but the majority possessed a distinct clear nuclear area contain- 
ing two disc-shaped greenish nucleoli. In some cases the denser 
portion of the body was crowded with an amorphous mass of 
granules, and in others similar granules were aggregated into 
spheres contained in fluid vacuoles (Fig. 22). In appearance 
Fic, 22.—Large Ameeba with digestive vacuoles x 1000. 
and measurements the granules within the Amcebz were identical 
with the bacterial spores of the medium. The Amebze precisely 
resembled those frequently encountered in fresh human excreta, 
but in size considerably exceeded those normally developed in 
cow dung cultivations. 
On the following day the cultivation was found to be crowded 
with huge active Amcebz, like those of the previous day. When 
first introduced into the nutritive fluid of the preparations they 
presented a peculiar tuberculate or irregularly cornuate outline, 
but they rapidly unfolded and crawled freely about. Their 
nucleoli varied greatly in size; in some cases the discs attained 
a diameter of 5°5 uw. Specimens of Amceebe which had been re- 
served beneath a cover-glass had passed into the condition of 
