382 DR. E. KLEIN. 
ultimate lymphatics. This is the method followed by 
Neumann (l.c¢., p. 8); “The method that I used was the 
modified method of Hyrtl-Teichmann ; the epidermis having 
been removed by maceration in a mixture of alcohol, acetic 
acid, and water, and also the rest of the tissue having become 
macerated, an incision half to one line deep is made with a 
pointed cataract needle, into which a fine tubule is fixed, 
and the injection through a fine brass syringe is proceeded 
with.....” As injection material Neumann used (I. ¢., p. 9) 
carmin-ammonia in conjunction with glycerine, and car- 
bonate of lead rubbed down in a little glycerine. On p. 10 
he informs us that to prevent mistaking the lymphatics for 
blood-vessels he injected the latter with Berlin blue. But, 
to our profound astonishment, we find in the beautiful plates 
accompanying his memoir, unexpectedly all lymphatics 
represented as if injected with Berlin blue, although this’ 
pigment, according to his statement, is reversed solely for 
the injection of the blood-vessels. These latter are repre- 
sented (in the plates) as if injected with carmin-ammonia ; 
but, according to his statement, this is the material which he 
used for the injection of the lymphatics. 
After this, and after his mentioning (1. c., p. 9) that it is 
advisable to stretch the skin that is to be injected over the 
left finger during each injection, we cannot expect that 
Neumann should have been able to trace the ultimate root- 
lets of the lymphatics. 
6. The method which I follow is the same in the case of 
the skin and mucous membranes; it is this: I always use 
the material as fresh as possible; my most successful speci- 
mens were obtained from skin and mucous membranes pre- 
pared immediately after death. This applies equally to the 
structures of man and animals. To use material that had 
been previously macerated in alcohol and acetic acid, as in 
the case of Neumann’s material, and to expect that the 
injection would reveal the finest rootlets, appears to me too 
sanguine. 
Human skin is best; that of the dog and rabbit is also 
good. Of the former the scalp, the skin of the face, of the 
lips, of the palm of the hand and that of the sole of the foot, 
are those parts that yielded the best preparations. 
The method of preparation is that of injection by “ punc- 
ture.” This is always performed on the skin 7m siti, not on 
skin cut out previously and stretched over the finger (!) as is 
done by Neumann. 
7. The injection material used is Bricke’s Berlin blue in 
a solution of two. to four per cent., or asphalt dissolved in 
