384 DR, E. KLEIN. 
presenting a uniform colour, brownish black if asphalt is 
used, blue with Berlin blue. 
The intensity of the colour and the swelling up of the 
injected skin is always greater in the former, i.e. asphalt, 
than in the latter case, ¢.e. Berlin blue. But of course this 
depends on the amount of the injected material and on the 
ease with which it spreads in the skin, for if a relatively 
large quantity is injected which does not spread into a com- 
paratively large area, the skin will be deeply coloured and 
it becomes very thick, owing to the lymphatics being greatly 
distended by, and filled with the injection matter. 
In the description of the appearances of the lymphatics, to 
be given below, we shall refer only to such preparations in 
which the injection has been effected with great ease, the 
skin and mucous membranes having become readily injected, 
and slightly but distinctly thicker than in the uninjected 
state; the lymphatics are then found in a moderate state of 
distension. 
In the case of the mucous membranes where the lym- 
phatics are easily recognised in the injected organs with the 
unaided eye, the successful progress of the injection becomes 
soon apparent for the vessels become suddenly filled over 
large areas. 
9. The skin and mucous membranes after the injection 
are hardened in dilute spirit: three parts of methylated 
spirit to one part of water; or in a mixture of two parts of 
+. per cent. watery solution of chromic acid and one part of 
methylated alcohol; or in } to + per cent. watery solution of 
chromic acid. When sufficiently hard, sections are made, 
stained, and mounted in the usual manner. 
There are, however, certain disadvantages connected with 
the use of the asphalt-benzole. In the first place it is not 
so easily managed as watery solutions, and this of course 
applies to all other similar materials dissolved in turpentine, 
chloroform, oil, &c. ; the cleaning of the syringes, canulas, 
tubes, and instruments require a deal of time and attention. 
In the second place the difficulties of mounting and pre- 
serving the sections of the injected skin are considerable. 
These can be of course mounted and preserved in glycerine 
without any difficulty, but the section lacks in transparency 
and in smoothness of appearance of the injected vessels. The 
sections cannot be preserved in Canada balsam or dammar 
varnish on account of the solubility of the asphalt in balsam, 
in turpentine, and chloroform. 
I have succeeded, however, to preserve specimens in a 
transparent state for several months in this way: the 
