4 PROFESSOR KUTIIERFORD. 



Every student provides himself with a box of slides and 

 cover- glasses, scissors, forceps, scalpels, razor, mounting clips, 

 needles in handles, camel-hair pencils, while he is furnished 

 with all the tissues, reagents, &c. I give every student a 

 stand with twelve one-ounce bottles, containing the reagents. 

 The bottles have good corks, in which are fixed glass rods, 

 sable, or camel-hair pencils. Some may say that corks are 

 bad in such a case. If expense were no object, of course 

 one would use Beale's excellent drop bottles, but they are 

 too expensive for class purposes, and really the corked bottles 

 do very Avell. I very rarely find a single cork cell in any 

 preparation. The fluids contained in the bottles are — 



1. Distilled water, so that a drop of clean water may be 

 had when necessary. 2. Solution of chloride of sodium 

 0"75 per cent. (7.5 grains dissolved in 1000 grains of water), 

 for treating delicate protoplasmic tissues. This is commonly 

 called salt solution. S. Absolute alcohol. 4. Oil of cloves. 

 5. Oil of turpentine. 6. Glycerine. 7. Acetic acid. 8. Weak 

 spirit (1 part methylated spirit, 3 parts distilled water). 9. 

 Solution of magenta for staining protoplasm. (See staining 

 of tissues.) 10. Saturated solution of ])otassiura acetate for 

 preserving osmic acid preparations. 11. Solution of Canada 

 balsam in turpentine.^ (Pure Canada balsam, dried till it is 

 hard and crystalline, and then dissolved in turpentine.) 12. 

 A thick solution of Dammar resin in benzole, as a luting. 

 (This dries rapidly enough, does not crack, does not " run in" 

 like that sepulchral abomination asphalt.) Other reagents 

 are only occasionally used, and are provided when required. 



At the beginning of the course a general account is given 

 of the microscope and accessory apparatus. The student is 

 instructed how to clean the instrument, hoAv to measure its 

 magnifying power, how to measure the size of an object, and 

 how to draw. After this every student is provided with his 

 microscope, and the regular work of demonstration begins. 

 From time to time, as the course advances, short accounts are 

 given of the preparation and preservation of objects, &c. 

 These are not entered into, however, until the student has 

 had a little experience of the effect of agents upon torulse, 

 blood-corpuscles, &c. 



' I have hitherto always employed tliis fluid for class purposes. In future 

 I nieau to use Dammar solution instead of it. See "How to Preserve 

 Tissues." 



