PUACTICAL UISTOLOGY FOR MEDICAL STUDENTS. 



Preparation of Tissues previous to Commencement of 

 THE Course. 



A month before the course begins it is necessary to prepare 

 some tissues — e.g., to soften bone, to harden nerve tissues, 

 &c. A cat is a convenient animal to take. Kill it by bleed- 

 ing during digestion ; inject 0*75 per cent, solution of common 

 salt in water into the pulmonary artery, aorta, and portal vein; 

 open the stomach, and Avash out the interior with the salt 

 solution ; inject the salt solution into the intestine in order to 

 wash out the chyme and foeces ; cut intestine into short 

 pieces ; ])lace it, half of the stomach, half of the liver, the 

 spleen, the lungs, in a jar containing a large quantity of \ 

 per cent, solution of chromic acid ; place the other half of 

 the stomach and liver in methylated spirit; take out brain 

 and spinal cord, and place them in methylated spirit (see "De- 

 monstration of Nerve Tissue"); pierce the sclerotic with holes, 

 and put the eyes into Miiller's fluid. (See " How to Harden 

 Tissues.") Place the bones in the fluid recommended for 

 softening bone. (See " How to Soften Tissues.") When all 

 those tissues are hard enough, and when the bone is soft 

 enough, place them in methylated spirit until seel ions are 

 to be made. 



Demonstration of Tissues prepared by Each Student. 



The following is the order and the nature of the work 

 undertaken by my students. There is no difficulty in their 

 executing it in the number of lessons above mentioned. To 

 avoid repetition, let it be understood that mensuration and 

 drawing are practised Avhenever it is possible. 



1. Germinating yeast} 



D 1.^ Examine different forms of the torulse. a. Add 

 magenta ; observe effects, h. Rupture the torulse. 



2. Penicillium? 



D 1. Submergedhyphse. a, Add magenta. 5,Rupture. 



* The idea of beginning with this I owe to my friend Professor Huxley. 



- D 1 means Demonstration 1. 



^ The germs of common mould must be sown a week or so beforehand 

 in tlie following fluid, a modification of Pasteur's fluid : 



Water 8376 parts 



Cane Sugar .... 15'0 „ 

 Ammonium Tartrate . . . 1"0 „ 

 Potassic Piiosphate . . . 0'2 „ 

 Calcic Phosphate . . . 0'02 „ 

 Magnesium Sulphate . , 0*02 „ 



