6 PROFESSOR RUTHERFORD. 



D 2. Aerial hyphae. a, Acid magenta ; observe hyphse, 

 gonidia. 



3. Hairs from stamens of Tradescantia virginica. Hairs 

 ill water ; observe the cells, wall, nucleus, protoplasm, vacu- 

 oles, and movements of the protoplasm, a, Heat, and ob- 

 serve effect, h, A hot stage suitable for roughly heating a 

 microscopic object maybe made of a plate of tin, 9x2^ 

 inches, with a small hole in the centre. The slide is laid 

 upon it and a spirit lamp is applied at the end of the 

 plate. For fine work, of course, Strieker's hot stage is 

 necessary. 



4. Vallisneria. Portion of leaf, to see cyclosis. Heat, if 

 the movement be sluggish. 



5. Nettle hair. Examine protoplasmic movements. 



6. Potato. Thin section ; examine starch, a, Add solu- 

 tion of iodine. 



7. Cotton fibres. Add iodine and sulphuric acid. 



8. hincn fibres. 



9. Section of corh. 



10. Disc-bearing tissue. Thin vertical section of cedar 

 pencil. 



11. Dust from some shelf. 



12. Gamboge, rubbed up in water for Brownian move- 

 ment. 



13. Bacteria, from infusion of muscular fibres. 



D 1. Examine movements, &c. b, Add magenta. 



D 2. a. Examine movements, b, Heat slide to boiling 



point, c. Examine the fluid again. 

 D 3. Another specimen ; add a solution of quinine. 



14. Infusoria. From old water of flowers, or infusion of 

 muscle. Add iodine or magenta solution. 



15. Amoeba. 



16. Blood of tieivt. White blood-corpuscles. Watch move- 

 ments ; heat if necessary. 



17. Cilia. 



D 1. Epithelium from trachea of a cat newly killed 

 by bleeding. Examine in salt solution. Observe 

 mucus-corpuscles, &c. 



D 2. Cilia from gill of salt-water mussel. Examine 

 in sea water, a, Apply heat. 



D 3. Mussel cilia, expose to vapour of chloroform. 

 Place cilia on under surface of a cover glass, 

 forming the roof of a putty cell, on the floor of 

 which a drop of chloroform has been placed. 



18. Cylindrical epithelium from intestine of the same 

 cat. 



