PRACTICAL HISTOLOGY FOR MEDICAL STUDENTS. 7 



Snip off villi. Examine in salt solution. Cylinder and goblet 

 cells. Add magenta. 



19. Glandular epithelium. Gastric follicles of the same cat 

 examined in salt solution. 



20. Squamous epithelium. Human saliva, a, Examine 

 epithelium and salivary cells, h, Add magenta. Observe 

 coloured nuclei and fibrils of coagulated ptyaline. 



21. Frog's hlood. 



D 1. Examine corpuscles, a, Add water to one side 



of drop, h, Add acetic acid to the other side. 



Observe effects. 

 D 2. Same blood. Add syrup or saturated solution of 



NaCl. 

 D 3. Same blood. Add magenta fluid, that especially 



used for staining blood. (See staining of tissues.) 

 D 4. Same blood. Add solution of tannic acid. (Two 



grains dissolved in one ounce of warm water, and 



allowed to cool.) Then add ordinary magenta fluid. 



(See staining of tissues.) 



22. Bird's hlood. Add magenta. 



23. Blood of fish. 



24. Human hlood. 



D 1. Examine corpuscles. Add water at one side of 



glass and acetic acid at the other. 

 D 2. Same blood. Add magenta. (Special fluid for 



staining blood — see staining of tissues.) 

 D 3. Same blood. Add tannic acid solution, and, 



after a time, ordinary magenta fluid. 



25. Blood of crah. 



26. Blood of guinea pig for haemoglobin crystals. 



27. Pus. Examine, and then add acetic acid. 



28. Mucus. Examine, and add acetic acid. 



29. Elastic tissue, Lig. nuchae of ox. 



Examine, a, Add acetic acid, alcohol, and preserve. 



30. A?'eolar tissue. Omentum from cat just killed. 

 D 1. Examine in salt solution, a, Add magenta. 

 D 2. Preserve in weak spirit. 



31. Adipose tissue, from omentum of the same cat. 

 D 1. In salt solution, preserve in glycerine. 



D 2. Adipose tissue which has been previously carmi- 

 nised so as to stain the nuclei of the fat-cells. (Ma- 

 genta does not stain these readily.) Preserve in 

 glycerine, to which hydrochloric acid has been 

 added. (See '•' How to Preserve Tissues.") 



32. Std)cutaneous areolar tissue from the same cat. 



D 1. a, Gently spread it out on dry glass, then add 



