8 PROFESSOR RUTHERIORD. 



salt solution and examine, h, Take off cover glass 

 and tear to pieces, in order to split up white fibres. 

 c, Add acetic acid. 



D 2. A new preparation for preservation in weak spirit. 



D 3. A new preparation for preservation in glycerine. 



33. Tendon. 



D 1. Transverse section of slightly dried tendon. 

 D 2. Vertical section of the same. Preserve in weak 

 spirit. 



34. Cartilage. 



D 1. White fibro-cartilage from intervertebral disc, in 



salt solution. Add magenta.^ Preserve in the 



following mixture : glycerine 1 part, water 2 parts. 

 D 2. Yellow fibro-cartilage from epiglottis. Preserve 



in the above dilute glycerine. 

 D 3. Hyaline cartilage. Section parallel with and 



near to the articular surface of articular cartilage. 



Preserve in dilute glycerine. 

 D 4. Vertical section of articular cartilage on head of 



a bone which has been softened. Preserve in dilute 



glycerine. 

 D 5. Section of costal cartilage of an old person. 



Preserve as above. 

 All these forms of cartilage may be previously macerated in 

 methylated spirit, and the sections mounted in Canada balsam. 

 (See " How to Preserve Tissues.") 



35. Bone. T. S.- and L. S. long bone of a cat previously 

 softened and preserved in methylated spirit. Mount in 

 methylated spirit. Return to the sections of costal and 

 articular cartilage, and examine them in this connection. 



36. Tooth. Show how sections of hard bone and tooth are 

 made. Send round a series of preparations of teeth and 

 different kinds of bone.^ 



37. Muscle. 



D 1. Boiled mutton or muscle of rabbit or cat. (The 



muscle must be fresh before being boiled.) 

 D 2. Boiled fish. 



' Magenta fades. Carmine must be used for things that are to be permanent. 



- T. S. means transverse section; V. S., vertical section; L. S., longitu- 

 dinal section. 



3 Such a series is previously arranged in order; those for a low and 

 those for a high power are placed in different groups. Every preparation 

 has a label mentioning all the points to be observed. When for a low power 

 it is marked L. ; if for a high power it is marked II. The preparations are 

 given to the students in order. They are questioned regarding them, and 

 when sufficient time has been allowed for the examination of each, a bell is 

 rung, and every man passes his preparation on to his neighbour. 



