PRACTICAL HISTOLOGY FOR MEDICAL STUDENTS. 9 



D 3. Boiled crab. 



Preserve all these in glycerine, glycerine jelly, or 

 weak spirit. 



D. 4. Fresh muscle of frog. Dissect it with needles in 

 water, in order to see sarcolemma. Add acetic acid. 



D 5. Muscle of frog previously carminised. Preserve 

 in acid glycerine. 



D 6. Muscle of frog or cat, which has been macerated 

 in dilute nitric acid (1 of acid to 4 water) for a 

 week or so. Squeeze fibres in order to get fibrils 

 and discs. Preserve in spirit or glycerine jelly. 



D 7. Fresh muscle from leg of water beetle in salt 

 solution, to see contraction waves, fibrils, and nerve 

 endings. 



D 8. Non-striped muscle from boiled stomach. Pre- 

 serve in spirit or glycerine jelly. 



D 9. Give each student a small piece of carminised 

 frog's bladder. Preserve in acid glycerine. 

 •38. Blood-vessels. Brain of a sheep cut into small pieces 

 and macerated for two days in glycerine. 



D 1. Strip off pia mater. Wash in water with the aid 

 of a camel-hair pencil. Add acetic acid. Preserve 

 in glycerine. 



D 2. A new preparation. Add no acid. Preserve 

 in glycerine jelly. 



D 3. Stain blood-vessels of frog's mesentery with nitrate 

 of silver (see staining of tissues), and examine it 

 next day. Preserve in glycerine jelly. 



D 4. Aorta of ox. T. S. with Valentin's knife. Pre- 

 serve in glycerine jelly. 

 Show how to inject, and send round a series of preparations 

 showing injected areolar tissue, bone, tooth, muscle, and 

 various arrangements of blood-vessels. 

 39. Nerve. 



D 1. Fresh nerve of frog, a, tease out with needles in 

 salt solution, and examine, h, Wash with water 

 and tease the nerve out in the water, and examine 

 again to see the fibrillation of the white substance. 



D 2. L. S. white matter of fresh spinal cord of sheep. 

 (Add no fluid.) 



D 3. White matter of fresh cerebrum of sheep. (Add 

 no fluid.) 



D 4. Cells of fresh Gasserian ganglion in salt solution. 

 Add magenta to colour cells and axial cylinders. 



D 5. Grey matter of cerebellum (fresh) to see cells of 

 inner grey layer. (Add no fluid.) 



