PRACTICAL HISTOLOGY FOR MEDICAL STUDENTS. 11 



D 3. Examine a series of lung preparations. 

 -t 1 . Alimentanj canal. 



D 1. T. S. hardened cat's tongue. 



D 2. V. S. hardened stomach. 



D 3. V. S. hardened small intestine of cat. 

 Clarify and preserve D 1, 2, and 3 with glycerine. 



D 4. Fresh small intestine of cat, killed during diges- 

 tion. Wash surface of mucous membrane with salt 

 solution. Snip off a few villi with scissors and 

 examine them. 



D 5. T. S. hardened large intestine of cat. TTreat as 

 in D 3.) 



Examine a series of preparations of alimentary canal. 



42. Liver. 



D 1. Cells of fresh cat or rabbit's liver. Examined 



in salt solution. Add magenta. 

 D 2. Cells of liver of ox, examined in the same way. 



These are usually fatty. 

 D 3. Thin section of liver of cat hardened in chromic 

 acid or alcohol. Clarify with, and preserve in, 

 glycerine. 

 Examine a series of preparations of injected liver, salivary 

 glands, and pancreas. 



43. Kidney. 



D 1. V. S, with Valentin's knife Malpighian pyramid 

 of fresh sheep's kidney, examined in salt solution. 

 a, Examine with low power, h, Take off covering 

 glass and dissect with needles a portion of the 

 cortical and a portion of the medullary substance, 

 c. Examine with high power, d, It may be neces- 

 says to wash the section with salt solution ; use a 

 camel-hair pencil for this purpose, e. Add magenta, 

 and observe the action upon the epithelium of both 

 straight and convoluted tubules, and the effect upon 

 the matrix. 



D 2. T. S. of frozen Malpighian pyramid near its 

 apex. (This may also be made from a fresh kidney 

 with Valentin's knife.) 



D 3. Scrape the cut surface of the cortical substance, 

 in order to get isolated Malpighian bodies, ^^.dd 

 acetic acid. 



D 4. Give each student a thin vertical section of the 

 Malpighian pyramid of a pig's kidney, Avhich has 

 been macerated for eight days in \ per cent, solu- 

 tion of potassium bichromate, and washed in water 

 for fifteen minutes (Klein). Mount in glycerine. 



