RHINOSPORIDIUM KINEALYI. wo 
namely the contents of the cysts. They have been described 
by O’Kinealy (2) as granular bodies, containing refractile 
granules. Vaughan (1) has termed the granular bodies 
“sporules,” but for descriptive purposes we will commence 
by employing the non-committal terms used by O’Kinealy. 
If one of the larger cysts be examined, it is at once obvious 
that the spherical or ovoid granular bodies vary greatly in 
size, Showing every gradation from small bodies at the peri- 
phery to larger ones towards the centre, and, further, that 
the number of refractile granules in the bodies is also subject. 
to great variation (figs.6 and 7). For purposes of description 
it is convenient to consider the granular bodies as forming 
three zones or regions, namely peripheral, intermediate, and 
central, although in reality no hard and fast line can be drawn 
between these three categories. In preparations stained 
with safranin and licht-griin, however, the peripheral bodies 
stain green, the central ones red. ‘I'he peripheral granular 
bodies measure about 1—1°5 » in diameter, and contain a single 
refractile granule or nucleus (figs. 6 and 7). Those of the 
intermediate zone measure 2—2°5 uw in diameter, and contain 
two or three granules in addition to a distinct nucleus (figs. 6 
and 7). ‘This structure can be made out in a preparation 
staimed by Heidenhain’s method, in which the body appears 
to contain one nucleus and several granules in the form of 
more or less concentrated spherical masses of protoplasm 
(fig. 7), though in the elucidation of these structures in the 
intermediate zone thinner sections would have been most 
helpful, indeed desirable. ‘the central granular bodies 
measure 5—6 w in diameter and contain from 9 to 15 refractile 
granules. In the original picrocarmine preparation each 
refractile granule shows distinctly a central spot of different 
refringence from the surrounding part, and appearing darker 
or lighter according to the focus of the microscope. In 
preparations made by Heidenhain’s method the central spot 
takes up the hematoxylin intensely and then appears in many 
cases deep black when carefully focussed. Delafield’s hama- 
toxylin also stains the central spot, though not so well. From 
