526 E. A. MINCHIN AND H. B. FANTHAM. 
these reactions there can be no doubt that the central spot of 
the so-called refractile granules is a chromatic nucleus. Hach 
granular body is thus seen to be a morula-like structure, 
containing on the average about a dozen nucleated corpuscles, 
for which we may now drop the term “ refractile granules” 
and call them simply spores. Each spore-morula is bounded 
by a membrane, which in the original picrocarmine pre- 
paration appears highly refractile. Stained by Heidenhain’s 
method, the membrane in question is still refractile but more 
distinct, while after Delafield’s hematoxylin it is very clearly 
seen and darkly stained, though we have not been able 
to make out the ‘‘ double fine contour line” described by 
Vaughan. The spore-morule appear more closely apposed and 
facetted in the iron-hematoxylin preparations, but slightly 
separated from one another in the slides stained with picro- 
carmine and Delafield’s hematoxylin; either as the result of 
swelling in the former case, or shrinkage in the latter. Another 
curious point about the spore-morula, seen in the original 
picrocarmine preparations, is that some of them take up more 
of the carmine and have a red tinge, while the majority stain 
a light yellow colour; the former are apparently the fully 
developed, older bodies. Within the morula the spores have 
a more or less irregular arrangement (fig. 8). 
‘he above description refers to what may be considered as 
a typical, fully developed cyst, and applies to the majority of 
the cysts in the sections we have studied. Besides these, 
however, we have found others measuring about 120 w in 
diameter, less rounded in shape, in which no spore-morule 
are to be found, but the entire cyst is packed with spherical 
masses of protoplasm, each containing a single nucleus (fig. 5), 
In other words, the “granular bodies” in these cysts have 
not advanced beyond the condition of the peripheral zone in 
the cysts described above, and there can be no doubt that we 
are dealing with younger or immature cysts. Another very 
important feature of these smaller cysts is the presence of a 
peripheral zone of clear undifferentiated protoplasm imme- 
diately within the cyst-wall (fig. 5). We have searched care- 
