STRUCTURE, ETC., OF PLEISTOPHORA PERIPLANETM. 681 
reticulated and coloured a bright red, the less numerous compact and of a 
purplish tint. In the middle of the trophozoite an aggregation of these purple 
and probably degenerated nuclei is to be seen. The presence of these nuclei 
probably indicates approaching sporulation. 
Fre. 10.—Another trophozoite of similar character. 
Fie. 1].—An example of simple plasmotomy. A trophozoite is in the last 
stage of binary fission. 
Fig. 12.—Small trophozoite showing the early occurrence of the purple 
nuclei. 
Fic. 13.—Large trophozoites undergoing multiple plasmotomy, or, in other 
words, budding off small multinucleated masses of protoplasm, which will 
become independent trophozoites. One bud is already detached. 
Fre. 14.—Pansporoblast at an early stage of development. Each of the 
oval sporoblasts appears to be lying in a vacuole. This appearance is pro- 
duced by the shrinkage which the protoplasm undergoes in preparation. The 
nuclei of the sporoblasts are seen to be diffuse and reticular. The interstitial 
or residual nuclei retain in many cases the bright red tint of the normal 
nucleus. Two can be seen to be purplish. Fig. 30 represents a pansporoblast 
at a very slightly earlier stage. 
Fie. 15.—Nucleus of cell of Malpighian tubule fixed by drying and showing 
the red colour assumed by nuclei, when stained by Giemsa. The figure should 
be compared with Fig. 27, which represents a similar nucleus fixed, however, 
with corrosive sublimate and alcohol, and stained with Delafield’s hema- 
toxylin. 
Fic. 16.—Multiple amitosis; trophozoite about to break up into four 
sporozoites. 
Fic. 17.—Similar figure, but nuclei more numerous. 
Fic. 18.—Later stages of same process. 
Fic. 19.—Group of sporozoites just after division. 
_ Fic. 20.—Large trophozoite budding off unenucleated spores. 
Fic. 21.—Trophozoite of hitherto undescribed Myxosporidian with the 
same habitat as Pleistophora periplanete. The nuclei are compact 
and much smaller than the nuclei of the Pleistophora. 
Fig. 22.—Trophozoite and group of spores of the same Myxosporidian. 
Several of the nuclei undergoing binary fission. 
Fie. 23.—Trophozoite of same. 
Fic. 24.—Trophozoite of same, the nuclei being reticulated and surrounded 
by a vacuole, 
Fies. 50 and 51.—Small trophozoites dividing into two. 
