62 DR. E. KLEIN. 



inoculation there are a few haemorrhagic spots, but the oedema 

 is generally free of blood ; cutting into the tissue a quantity of 

 clear serum flows out that contains only very few bacilli, if 

 examined soon after death. Later on their number is much 

 increased. In some cases, especially of prolonged illness, this 

 oedema is so extensive that the subcutaneous tissue of the in- 

 guinal region, abdomen, and chest is uniformly infiltrated with 

 the serum. This oedema I have not missed, with very few ex- 

 ceptions, in the cases of inoculation of Bacillus anthracis 

 and their spores, no matter whence derived, if the inoculation 

 is made into the subcutaneous tissue of the inguinal or abdo- 

 minal region, but it was absent if that of the ear-lobe was the 

 seat of inoculation. That in our case we have to do with 

 veritable anthrax there can be no manner of doubt from the 

 symptoms, the nature of the bacilli, and their distribution in 

 the organs. Pasteur mentions a similar appearance of oedema 

 in some of his sheep. 



In rabbits the oedema is not so frequent as in guinea-pigs, nor 

 is it so pronounced, but nevertheless I have met with it in several 

 instances of inoculation into the subcutaneous tissue of the 

 inguinal region.' 



It must be, however, understood that this symptom of sub- 

 cutaneous oedema in guinea-pigs after inoculation into the 

 subcutaneous tissue of the inguinal region occurs not only after 

 the inoculation with blood or with tissues, but equally distinct 

 with artificially cultivated bacillus and its spores. 



Inoculation into the corium itself of the inguinal region of 

 guinea-pigs produced only very slight oedematous swelling about 

 the point of inoculation ; in rabbits such an inoculation is not 

 associated with oedema. 



2. Inoculations with artificially cultivated Bacillus 

 a nthracis. 



' What the meaning of the statement of Wernich's (' Central f. med. Wiss.,' 

 No. 12, 1882, p. 217) is, that rabbits, "although not absolutely refractory, 

 nevertheless are very little susceptible " to anthrax, I cannot comprehend, 

 since 1 have never found a rabbit escape death after inoculation with anthrax 

 blood or artificially cultivated active Bacillus anthracis. 



