278 H. MARSHALL WAliD. 



The future behaviour of these zoospores — -for such they 

 must be considered — may be described, as before, from what 

 was observed in a given example. Careful examination of one 

 of the more loosely attached specimens on the outer portions 

 of the spherical group (figs. 3 and 4) convinces the observer 

 that an extremely delicate envelope becomes developed at the 

 periphery of the resting globular zoospoore (fig. 5 a), and the 

 body remains in this condition for some hours. 



The specimen referred to was drawn at 1 p.m., and remained 

 in this condition until about 4 p.m. Soon after this there were 

 signs of change going on in the neighbouring specimens, and 

 this particular globe was carefully watched. At 4.14 a slight 

 protuberance made its appearance at one side, rapidly increased 

 in size during the next minute or two (fig. 5 b — d), and a clear 

 space (e) was then seen separating the delicate envelope from 

 the granular, slightly amoeboid protoplasmic contents, which 

 were, in fact, becoming withdrawn to pass through to the outside. 

 A minute afterwards the whole of the protoplasm was outside, 

 except a minute papilla, which slipped out forthwith (fig. 5f, g), 

 and. the mass commenced to writhe slowly in an amoeboid 

 manner outside the very delicate empty envelope, in the side 

 of which could be seen the minute pore through which the zoo- 

 spore had slipped out. At 4.18, the moment of complete exit, 

 a clear spherical vacuole was seen at one side of the zoospore 

 (g) ; the latter then quickly acquired a reniform shape, and 

 from the sinus (corresponding to the hilus of the kidney), two 

 minute cilia with knobbed tips were observed to spring forth, 

 quickly grow in length, apparently at the expense of the knobs 

 at their ends, and begin to wave slowly about. The zoospore 

 noAV (4.25) commenced to swing perceptibly as the lashing of 

 the cilia became more vigorous; nevertheless it did not move 

 away for some time ; at 4.35, however, the zoospore was freely 

 and rapidly moving about, and at once disappeared from the 

 field. 



This process, described as faithfully as possible in one case, 

 was repeated by the contents of the rest of the spheres com- 

 posing the globular mass at the mouth of the zoosporangium 



