296 Dll. VINCENT HARRIS. 



considered, but no doubt the effects of the staining fluids were 

 not constant. 



Red and blue. (Fuchsin and methylen blue.) 

 Certainly one of the most successful combinations. The 

 methylin blue was used as a saturated solution in absolute 

 alcohol. In this case, as in most of the others, the blue was 

 used second to the fuchsin, and vice versa , with similar 

 results. In these specimens the nuclei of the coloured cor- 

 puscles were deeply stained blue. Of the remainder of the 

 corpuscle, a light greenish hue with the edge a bright pink, or 

 where the staining had been less deep the whole of the cor- 

 puscle, except the nucleus, was stained pink with an edge of 

 a deeper but similar colour. The staining of the colourless 

 corpuscles was peculiar ; some were alight bluish green. These 

 were irregular and branched ; others had a deeper purple 

 colour with unstained spots (vacuoles ?) ; and a third variety 

 appeared to be stained in two colours ; in these a large central 

 mass looked like an immense nucleus. Other varieties might 

 be mentioned, as in the last combination, but as it is possible 

 that the size, amount of granules, and the staining are, except 

 in the three varieties, mere differences in amount not in kind, 

 it is scarcely necessary to mention them. For purposes of 

 demonstrating the divisions, irregularities in shape and in 

 varieties of colourless corpuscles, I am strongly inclined to 

 recommend staining with methylen blue and fuchsin as the 

 best combination possible. 



Hed and blue, 2nil combination. (Fuchsin and soluble 

 anilin blue.) 



Nuclei stained red, as well as the colourless corpuscles ; 

 stroma a light blue. It is as good a combination as the last. 

 Red and violet. (Eosin and methyl violet.) 

 AVith these colours there appeared to be a mixture of the 

 dyes in the corpuscle, and the nuclei were not distinct. 

 Red and brown. (Fuchsin and Bismarck Brown.) 

 Bismarck brown is an anilin dye of considerable utility ; it 

 is partially soluble in water, more so in water with a few 

 drops of glycerin added to it, and easily soluble in dilute 



