DOUBLE STAINING NUCLEATED BLOOD-CORPUSCLES. 297 



methylated spirit. The solution used was 2 per cent, in 

 dilute spirit. The corpuscles stained easily and in a fairly 

 regular manner, the nucleus a deep red, the stroma a fine 

 brown. The colourless corpuscles pinkish red. In a few 

 eases I noticed a mixture of the colours in the corpuscles. 

 In using Bismarck brown, a dye much employed on the Con- 

 tinent, I find it best to immerse the specimens in it for twenty 

 to thirty hours, and then they will retain their colour even 

 if passed slowly through the dehydrating and clearing fluids. 



Red and brown, 2nd combination. (Eosin and ve- 

 suvin.) Vesuvin and Bismarck Brown are said to have the same 

 chemical formula, and are probably identical. 



The vesuvin was used in a strong aqueous solution. The 

 corpuscles easily stained with eosin, nuclei and colourless 

 corpuscles a deep pinkish colour, and the stroma of the 

 coloured corpuscles a light pink. After double staining with 

 vesuvin the stroma stained a light yellowish brown, leaving 

 the nuclei and the colourless corpuscles stained as before. A 

 very successful combination. 



Series B. — Primary colour green ; tried with brown, red, 

 orange, yellow, blue, and violet. 



1st Dye. 2nd Dyes. 



Substances used. Substances used. 



Green — Iodine or Malachite Brown — Bismarck. 



Green. Red — Flamingo or Ponceau. 



Orange — Aurinand Auilin Orange. 

 Yellow — Anilin Primrose. 

 Blue — Bleu de Lyon. 

 Violet— Methyl Violet, 



Series B. 

 Green and brown. (Iodine green and Bismarck brown.) 

 The colourless corpuscles and the nuclei of the coloured 

 corpuscles distinctly green ; the stroma brown. 

 Green and red. (Iodine green and flamingo.) 

 Flamingo, a deep brownish red, soluble in water partly, but 

 freely in dilute spirit. The latter solution was the one em- 

 ployed. The nuclei of the coloured and the whole of the 



