298 DR. VINCENT HARRIS. 



colourless corpuscle showed a deep bluish green, and the stroma 

 was coloured pink. 



Green and red, 2nd combination. (Malachite green and 

 ponceau.) 



Malachite green is freely soluble in water, and was used in 

 aqueous solution. The nuclei of the coloured corpuscles 

 stained green and the stroma a light pink. The colourless 

 corpuscles of the same shade as the nuclei. On keeping the 

 specimens it was found that the green dye almost entirely 

 disappeared. 



Green and orange. (Malachite green and fluorescin.) 

 The nuclei of the coloured corpuscles stained a light yel- 

 lowish green, as did also the colourless corpuscles. The 

 stroma stained a yellow colour. The green stain was very 

 temporary. 



Green and orange. (Malachite green and aurin.) 

 Entirely unsuccessful. 



Green and yellow. (Iodine green and anilin primrose.) 

 The colourless corpuscles and the nuclei of coloured cor- 

 puscles stained green ; the stroma of the latter yellow. 

 Green and blue. (Iodine green and Bleu de Lyon.) 

 Double staining quite unsuccessful. The Bleu de Lyon was 

 employed in a dilute spirit solution. 



Green and violet. (Malachite green and methyl violet.) 

 Combination not good. The nuclei stained a very light 

 purple, as did also the colourless corpuscles, whilst the stroma 

 was a pinkish yellow. The green and violet apparently 

 mingled in staining the nuclei. 



Series C. — Primary colour, violet; tried with brown, red, 

 orange, yellow, green, and blue. 



1st Dye. 2nd Dyes. 



Substances used. Substances used. 



Violet — Brown — Bismarck. 



Methyl Violet. lied — Elamiugo, Eosin, Aniliu Scarlet. 



Gentian Violet. Orange — Tropffiolin. 

 Hoffman's Violet. Yellow — Anilin Primrose. 

 Green — Iodine Green. 

 Blue— Methylen Blue. 



