DOUBLE STAINING NUCLEATED BLOOD-CORPUSCLES. 299 



Violet and brown. (Hoffman's violet and Bismarck 

 brown.) 



An excellent combination. An aqueous solution of the 

 violet was used, and a dilute spirit solution of the Bismarck, 

 brown. The result showed excellent double staining. The 

 nuclei of the coloured corpuscles and the colourless corpuscles 

 were stained a reddish brown, and the stroma a light brown. 

 The brown evidently stained the whole of the corpuscle 

 stroma, and nucleus, but met the violet in the nucleus, and 

 together stained it the colour it presented. 



Violet and red. (Hoffman's violet and flamingo.) Fla- 

 mingo is a mixture of rosanilin and Bismarck brown. 



The nuclei and stroma stain-ed two shades of the same colour, 

 probably a mixture of the two used, mauve. 



Violet and red, 2nd combination. (Gentian violet and 

 anilin scarlet.) 



Unsuccessful, 



Violet and red, 3rd combination. (Gentian violet and 

 eosin.) 



Nuclei of coloured corpuscles, deeply stained red, with the 

 colourless corpuscles a similar colour with their nuclei. Stroma 

 a light pink. 



Violet and orange. (Hoffman's violet and tropaeolin.) 



Tropaeolin used in 1 % watery solution. Double staining 

 entirely failed. 



Violet and yellow. (Gentian violet and anilin primrose). 



Strangely enough the corpuscles were stained two shades of 

 green. 



Violet and blue. (Methyl violet and methylen blue.) 



Certainly one of the best combinations tried. The methyl 

 violet is a very pink dye, and the blue a very deep blue. The 

 latter stained the nuclei, the former the stroma. 



Results. — From my experiments I draw the following conclu- 

 sions as to double anilin staining of the nucleated corpuscles. 

 It seems reasonable to look upon such corpuscles as made up of 

 only a few varieties of tissue, and as such I have spoken of 

 them. 



