ON BUDDING IN POLYZOA. 521 



&c.). JoHet also witnessed the ingestion of the brown body in 

 Eucratea chelata, which passed through the alimentary 

 canal of the developing polypide, but owing to its resistant 

 membrane the brown body suffered no alteration ; but in 

 Lepralia granifera the very thin envelope of the brown body 

 is destroyed, not being able to resist the action of the juices of 

 the stomach, the movements caused by the cilia, and the con- 

 tractions of the intestinal walls : thus the brown granules 

 which it contains are set at liberty, whirled about and shortly 

 evacuated by the rectum. Hincks, himself (I.e. p. Ixii, footnote) 

 noticed the formation of a polypide-bud quite separate from the 

 brown body in Bugula calathus. 



After the able discussion of the subject by Joliet (17), it 

 seems quite superfluous to reopen the controversy as to the 

 probable origin of the bud from the brown -body ("germ- 

 capsule"); but Hincks (1. c. p. Ixiii) has still left it a slightly 

 open question. According to Smitt (and Hincks), there would 

 be at least two modes of bud-formation amongst the Polyzoa: 

 1. In the old zooecia {a) formed quite close to the brown-body, 

 and arising directly from it, {h) formed at a distance from the 

 brown-body and not arising from it. 2, In the new zooecia, 

 also arising de novo. From the accounts of other observers, 

 one method of bud-formation serves in all cases, the origin 

 in an old or a new zooecium being always from the same 

 tissue, though they are by no means agreed as to what that 

 tissue is. It is merely a question as to how close to or how far 

 from the brown-body the bud shall arise. 



Taking all the evidence we possess, it seems to be quite 

 evident that the generally received account is the correct one, 

 but that the approximation of the undeveloped bud to the 

 brown-body may mask its real distinctness in a few instances. 



A further observation on a living specimen (Naples, Sept., 

 1881) is represented on PI. XXVIII, fig. 13. The bud had 

 reached the stage of PI. XXXVII, fig. 1; it was suspended in 

 the anterior band of endocyst, and was connected with the 

 parent polypide by the tentacular sheath of the latter, and 

 probably also by some funicular tissue. In this example the 



