574 0. A. MAO MUNN. 



belong to the animai or the algae. I have already^ proved this 

 point almost completely, as I found that iuAnthea cereus, 

 in Bunodes Ballii, and Sagartia Be His, '^yellow cells/^ 

 or symbiotic algse, are present, that these animals all contain 

 chlorofucin, all contain the same accompanying colouring 

 matters, and that these colouring matters are evidently due to 

 the " yellow cells " with which the tentacles are stuflFed ; for 

 there is no essential difference in the spectra of the solutions of 

 the tentacles in which the colouring matters are derived entirely 

 from the " yellow cells " and those obtainable from other parts 

 of the Actinia. 



Moreover, I have also proved that in anemones possessing 

 yellow cells there is more or less suppression of the respiratory 

 proteids found in other Actiniae. 



But I had not repeated Sorby^s experiments in which he 

 applied Stokes^s "fractional" method for the separation of the 

 chlorofucin from the other colouring matters. In the present 

 paper I have given the results of this examination, and, as 

 will be seen, the statements of Sorby have been verified. This 

 is of importance, as Krukenberg" has omitted to mention in the 

 account of his experiments the results arrived at by Sorby, 

 although, as I shall show, he had evidently chlorofucin before 

 him in some of the solutions whose spectra he has mapped. 



In the paper referred to above^ I have shown that the mixture of 

 colouring matters obtained from the Actiniae therein mentioned 

 contain chlorofucin, and that the bands of this correspond to 

 the chlorofucin bands in a similar solution of Fucus serratus. 

 Sorby has figured in a diagram the bands of this pigment, but he 

 does not give their wave-length measurements, and only figures 

 the dominant bands of " blue '' and " yellow chlorophyll " in 

 the same diagram for the sake of comparison ; consequently- 

 some confusion is caused when one endeavours to find out what 



1 'Proc. Roy. Soc.,' No. 235, 1885, and 'Philos. Trans.,' 1885, Part II, 

 641, and seq. 



« ' Vergleichend-Physiol. Studien.,' Iste Reihe, 5te Abtli., 1881, S. 38, 

 and Ibid. 2te Reihe, 3tte Abth., 1882, S. 72. 



3 • Philos. Trans.,' loc, cit. 



