580 C. A. MAC MUNN. 



good daylight. The presence of the second band (commencing 

 from the red end) at once stamps this spectrum and distin- 

 guishes it from the usual chlorophyll spectrum. These bands 

 read approximately : 



1st band ... X 674-5 to \ 653. 

 2iid „ ... X 641 to X 625. 

 3rd „ ... X 595 to X 575. 



While the 4th band was guessed to be A 467 to X 443; but 

 its edges were so ill-defined and it was so encroached upon by 

 the general absorption of the violet end that this measure- 

 ment may require to be corrected. 



The colour of solution (2) was a deep orange and it had also 

 a red fluorescence, but not so well marked as that of the 

 former solution. The spectrum is shown in sp. 3, and it is 

 seen that while the same bands are present as those of sp. 1, 

 yet the two first are of relatively different intensity 

 of shading. This points to the obvious conclusion that two 

 colouring matters are present, the one indicated by the band 

 in red, the other by the second band (and the third, as will be 

 shown further on), there is more of the former present in the 

 solution of the tentacles, of the latter in that of the other 

 parts. The readings of these bands were the same as the last. 

 In a thinner layer of this solution (2) there are two bands 

 nearer the violet, that nearer the red being less shaded than 

 the other. Sp. 4 is an attempt to show this, but it only 

 approximately represents the position of these bands, of which 

 one is coincident with the lipochrome band of sp. 2. This dif- 

 ference of spectrum explains the diff'erence of colour of these 

 solutions, for the latter solution probably contains an additional 

 yellow colouring matter probably derived from the animal, 

 but it is not an additional green but a yellow. 



Solution (1) (of the tentacles) was now agitated in a sepa- 

 rating funnel, after dilution with water, with carbon bisul- 

 phide, when the bisulphide fell to the bottom of a yellow-green 

 colour leaving the alcohol layer orange. This bisulphide 

 solution on separating possessed a red fluorescence and gave 

 sp. 5, and the following readings : 



