NOTES ON THE CHROMATOLOGY OF ANTHEA OEREUS. 581 



1st baud . . . \ 6S5 to X 656. 



2tid „ ... X 647 to \ 612-5. (?) 



3rd „ ... X 597 to X 573. 



Two other bands in the green were also seen which doubt- 

 less are obscured in the alcohol solution by the shading 

 produced by the yellow constituents absorbing the violet end ; 

 and two others in the violet end were also visible^ which^ as far 

 as I could judge, measured approximately from X 479 to A 460, 

 and perhaps X 453 to X 436. 



A second bisulphide extract of the same solution, after the 

 first had been removed, was not nearly as green as the first 

 bisulphide extract, and contained less of the constituents 

 giving the bands in the red half of the spectrum, but showed a 

 dark band in the blue which was coincident with the X 479 to 

 X 460 band just mentioned. This spectrum is shown in sp. 6.^ 

 In every other respect the bands of sp. 5 and 6 agree. 



A third bisulphide extract of the same solution was hardly 

 coloured, and gave traces of the same bands. The alcohol 

 solution, which had been thrice extracted with bisulphide, was 

 of an orange colour an d contained a good deal of bisulphide, 

 and now this solution no longer showed the first band 

 in red, but did show the second and third bands of the original 

 spectrum; these latter are evidently the bands of Sorby's 

 chlorofucin, as can be seen by a comparison with his dia- 

 gram. They are shown in sp. 7 j in a thinner layer there are 

 other bands nearer the violet measuring approximately X 511 to 

 X 488, and X 477 to X 457, as shown in sp. 8. 



To see whether these two latter were the bands of Sorby's 

 fucoxanthin, a c ouple of drops of ammonia were added and a 

 little water ; the bisulphide layer was now turbid and gave one 

 broad band in green, the solution having an amber tint. (It 

 is noticeable that in this and in a second similar bisulphide 

 extract the dark bands in the blue and violet of sp. 6 and 8 

 were not present ; but the blue colour with hydrochloric acid 



' Possibljr the band may not belong to a lipochrome, as I found a pigment 

 in Authea's tentacles soluble in gljcerine, with a band in this part of the 

 spcctnnn. Sec below. 



