582 0. A. MAC MUNN. 



described by Sorby was not produced^ because this pigment was 

 not fucoxanthin.) The chlorofucin bands of sp. 7 after the bisul- 

 phide had removed as much colouring matter as possible was 

 (approximately) : 



1st baud . . . \ 644 to X 627-5. 

 Slid „ . . . \ 595 to X 581. 



There were, however, other bands present — one in blue 

 green and one in violet. This solution was a deep yellow 

 colour, due, probably, partly to the incomplete separation of 

 the yellow pigment giving the bands in the violet end. 



On evaporation of this solution some brown-yellow flocks 

 separated out ; the residue was extracted with absolute alcohol. 

 In this it formed a fine deep yellow solution, and gave sp. 7 

 again, the bands reading : 



1st band . . . X 641 to X 627-5. 

 2Dd „ ... X 595 to X 579. 



What the alcohol left undissolved was nearly all taken up by 

 distilled water, forming a yellow solution, giving some shading 

 at the blue end of green and absorbing the violet end. Hydro- 

 chloric acid did not seem to affect this solution. 



According to Sorby,^ the alcohol solution of chlorofucin is 

 changed by hydrochloric acid, and on adding this reagent to 

 its alcohol solution a new spectrum appears, namely, sp. 9, 

 whose bands read approximately : 



1st band ... X 607*5 to X 597. 

 2nd „ ... X 585 to X 573. 



Hence there is no doubt that this colouring matter was 

 chlorofucin. 



Solution (2) (i. e. the alcohol solution of the parts of Anthea 

 without tentacles) also contained chlorofucin, as I proved, by a 

 similar method. It was stated above that the solution (1), 

 from which the bisulphide had removed as much of the colouring 

 matter as it could take up, had been treated with ammonia 

 (two drops) and agitated afresh with bisulphide, and that this 



1 Loc. cit., p. 455. 



