NOTES ON THK CHROMATOLOGY OP ANTHEA OEREUS. 583 



bisulphide gave a baud in the green; such a solution was 

 evaporated to dryness, and the residue tested by the lipo- 

 chrome tests. With iodine and iodide of potassium it re- 

 mained unchanged, with nitric acid it gave a transient blue, 

 with sulphuric acid a green and blue ; hence it is a lipochrome, 

 A second absolute alcohol extract having been made from 

 the tentacles only was of a greenish-yellow colour with a 

 red fluorescence, and gave the following bands : 



1st band . . . \ 681-5 to \ 650 (dark part = X 678 to \ 653). 



2nd „ ... \ 636 to \ 601. 



3rd „ ... X 593 to X 573. (See Sp. 10.) 



In a thin layer there may have been some shading between 

 green and blue, and perhaps some in violet. To this solution 

 water was added, and it was then agitated with carbon bisul- 

 phide. On separating the latter it was greenish, leaving the 

 alcohol yellow, and in both these solutions certain bands in 

 the violet could now be seen, which were not visible in the 

 original alcohol solution (before separation). I have tried 

 to show all the bands in sp. 11 and 12. These read as 

 follows : 



Now, on comparing these bands with the bisulphide extract 

 from the first alcohol extract of the tentacles, they are found 

 to be practically almost the same. The yellow alcohol solution 

 from which the bisulphide had removed the pigment giving 

 these bands, no longer contained any chlorofucin, and showed 

 hardly a trace of a band in red in a deep layer. It did show 

 the shadow of a band in green, and perhaps another in the violet. 



If we compare a second absolute alcohol extract of the parts 

 of An the a free from tentacles with the second absolute extract 

 of the tentacles, we find that no great difference exists between 



VOL. XXVII, PART 4. NEW SER. S S 



