NOTES ON THE CHROMATOLOGY OP ANTHEA CEREUS. 585 



greenish colour with a blood-red fluorescence^ and gave the 

 following bands : 



1st band . . . \ 678 to \ 647. 

 2nd „ ... X 633 to X 601. 

 3rd „ ... X 591 to X 573. 



There was also a band at the blue end of green^ and perhaps 

 one in violet. Hence, then, the third extract of the tentacles and 

 the third extract of the parts without the tentacles contain the 

 same colouring matters. Now, the colouring matter in these 

 latter extracts, which gives the bands in the red end of the 

 spectrum, is almost, if not altogether, free from chlorofucin ; 

 at least the second and third bands of the spectrum give 

 different measurements. So far as I can judge this, then, 

 must be the pigment which Krukenberg would have belonging 

 to Anthea, which he calls ''Antheen,''' although I cannot 

 prove this assertion, owing to the way his spectra are repre- 

 sented. Krukenberg says that the corresponding pigment 

 withstands saponification, but in this case, as I have previously 

 shown,^ the addition of a little caustic soda alters the spectrum, 

 as shown in sp. 14.- 



A fourth absolute alcohol extract of the tentacles gave the 

 same spectrum, the bands reading : 



1st band . . . X 678 to X 650. 

 2nd „ ... X 633 to X 601. 

 3rd ,, ... X 591 to X 573. Others uncertain. 



A fourth similar extract of parts without tentacles read : 



1st band . . . X 678 to X 650. 

 2nd „ ... X 633 to X 601. 

 3rd „ ... X 591 to X 573. Others uncertain. 



And a fifth and sixth alcohol extract of the tentacles, and a 

 fifth and sixth of the parts without them, gave the same read- 

 ings. Hence we have precisely the same colouring 

 matters present in the tentacles as in the rest of the 

 animal, and as those in the tentacles are due to 



1 ' Philos. Trans./ Part II, 1885. 



^ NaHO causes precipitation, but after filtering, tlie solution is green, and 

 gives sp. 14. 



