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which were again successfully fertilized, and this partially 
replaced the lost lot, but it was noticed that this second set 
were not quite so mature as the previous ones, and the eggs 
did not run so freely from the parent fish. During the 
succeeding haul the entire net was carried away, and 
there being no spare one on board, we had, therefore, to 
return to Piel with only a small quantity of fertilized eggs 
as the result of two days’ fishing. 
“The police boat was awaiting our arrival outside the 
harbour, but the sea was too rough to board, so the 
‘Harriet’ had to bring us inside. Once inside the 
harbour, the embryos were conveyed ashore and trans- 
ferred to the tanks without further loss of time, the dead 
ones being first carefully removed. The embryos floated 
quite freely upon the surface of the water, and develop- 
ment followed its natural course, as could be seen by 
frequently examining the embryos under the microscope. 
“Probably owing to the eggs not being quite mature 
when obtained, and to injuries received subsequent to 
fertilization, a considerable daily mortality took place 
amongst the embryos, and at the end of nearly 200 
hours after fertilization, all had died and sunk to the 
bottom of the tanks. 
“ During the whole time that the embryos were under 
observation, the temperature and specific gravity of the 
water was taken daily. The temperature varied from 
5°8° C. to 6° C., and the specific gravity from °23 to ‘24. 
Although we were not fortunate in having the embryos 
hatch out at this stage of the experiment, it was clearly 
demonstrated that the specific gravity of the Piel water 
was sufficiently high to keep the eggs afloat. 
“At this point I had to return to Liverpool, but Mr. 
Wright, chief bailiff, was left in charge of the place with 
instructions to endeavour to obtain more fertilized eggs at 
