296 LILIAN J. GOULD. 
some were also stained by him. Others cut by Mr. Minchin 
were prepared by me, and together we obtained a most instruc- 
tive series, which I studied very carefully. The figures in the 
accompanying plate are from drawings I made with the camera 
under high powers, and illustrate the chief points dealt with in 
these notes. The stains used were Paul Mayer’s new “ carm- 
alum’? and ‘“ paracarmine” (‘Mitth. Neapel, Band x, 
1891-3, pp. 489, 491); anilines such as eosin, fuchsin, 
fuchsin S., orange G., and gentian violet, either alone or in com- 
bination; also picro-carmine and hematoxylin. The sections 
varied from 1 to 7 «in thickness. The only observations of mine 
which can claim to be new have reference (1) to the appear- 
ance of a central mass of doubtful significance in the general 
protoplasm, (2) to the staining properties of the refringent 
bodies or “ Glanzkérper” of Greeff, and (3) to the perfectly 
definite jointing staining-reaction of the rod-like bodies (a 
point suspected but hardly established hitherto), which 
establish the view that they are bacteria. These points, and 
others confirmatory of previous observations, are considered 
separately as follows. 
General Structure of the Protoplasm. 
The protoplasm of Pelomyxa is well known to be highly vacuo- 
lated, and in P. viridis Professor A. G. Bourne (‘ Quarterly 
Journal of Microscopical Science,’ xxxii, 1891) distinguishes 
between the large vacuoles, which are comparatively few, and 
the much more numerous smaller ones which he calls “ vesicles,” 
and which, in the species described by him, have chlorophyllo- 
genous contents. The difference in size, though not in colour, 
between ‘‘vacuoles” and “vesicles” obtains in P. palustris, 
and the numerical proportion of one to the other is much the 
same. Thus the general character of the protoplasm has been 
considered as practically identical in the two species. But I 
was able to make out that, in P. palustris at least, the proto- 
plasm surrounding the vesicles was by no means homogeneous, 
as stated by Professor Bourne for P. viridis, but showed 
very distinct structure. 
