MINUTE STRUCTURE OF PELOMYXA PALUSTRIS. 299 
specimen, and that the quiescent condition may have been pre- 
paratory to the production of swarm-spores. 
The outward appearance of quiescent individuals certainly 
seemed somewhat to resemble that described by Greeff (loc. 
cit.) as preceding reproduction. The protoplasm of all quies- 
cent individuals was filled with sand-particles to such an 
unusual extent that the cutting of sections was a matter of 
extreme difficulty. 
The “ Glanzkérper.” 
The refringent bodies, or “ Glanzkérper” of Greeff, were very 
numerous in P. palustris. Professor Bourne says he never 
saw “anything in the protoplasm of P. viridis resembling ” 
them, but it seems to me possible that he might have con- 
founded them with vacuoles of the same size, as in life they 
were often not easily distinguishable (except by the fact of their 
extrusion from the body) from these.1_ Nor were they always 
recognisable in sections when stains such ascarm-alum and picro- 
carmine were used. Alum-carmine, used by Professor Bourne, 
probably would not show them up either. But I found that 
they stained very readily and deeply with fuchsin, eosin, dahlia, 
solution of iodine in potassium iodide, picric acid dissolved in 
turpentine, and some other stains, and could thus be picked out 
with beautiful distinctness. With all stains except picric acid in 
turpentine they appeared perfectly homogeneous, but with the 
latter reagent they showed plainly a fine granulation, and 
sometimes contained a small bright crescentic area which 
might represent a space or cavity in the interior. From my 
observations I concluded that they were almost certainly either 
solid structures or filled with a coagulable fluid. Greeff 
observed the granulation with acetic acid, and found these 
bodies to become deep brown with iodine. I found a solution 
of iodine in potassium iodide to have the same effect. He also 
thought he could sometimes distinguish in the refringent bodies 
1 IT am inclined to regard the chlorophyll-bearing “ vesicles” of Bourne’s 
P. viridis as equivalent to the “ glanzkorper ” of P. palustris—E. R, L. 
