302 LILIAN J. GOULD. 
(fig. 4). I obtained the same result with a specimen treated 
in the same way, but stained with picro-carmine. In the sec- 
tions, which were mostly double- or treble-stained with anilines 
such as eosin, fuchsin, fuchsin S., orange G., and dahlia, the 
rods were deeply stained, and the jointing could be well seen ; 
in some cases the terminal joint could be seen separating off 
(fig. 7). The rods were always straight and made up of 2, 3, 4, 
6, and sometimes even 9 joints. They were highly refringent, 
and their refractive index seemed to be nearly the same as 
that of Canada balsam, since in preparations mounted in the 
latter medium the rods were difficult to see, while in glycerine 
they were most distinct. The joints had the shape of long 
prisms, and in a few of the 6-jointed rods a further transverse 
division of each joint into two was apparent. 
In the living Pelomyxa the rods were frequently thrown 
out into the water, together with refringent bodies and nuclei, 
a process evidently abnormal and the result of unfavorable 
conditions. When thus thrown out, the rods exhibited active 
movement of a kind which has been considered as possibly 
molecular (Bourne, loc. cit.), but they also travelled round 
the periphery of the animal. I could not absolutely satisfy 
myself that the latter movement might not have been due to 
currents created in the water by the activity of the pseudo- 
podia. Still, taking all the appearances together, it seemed 
impossible to doubt that the rods were really bacteria. 
My friend and fellow-student Mr. M. D. Hill, of New 
College, Oxford, undertook to prove this by cultivation of the 
bacteria in suitable media, and some account of his prelimi- 
nary investigations, which are not yet completed, are appended 
here. 
With regard to the situation of the bacteria, they were 
scattered more or less throughout the protoplasm, but were, 
as stated by Greeff, especially abundant around and adhered 
thickly to the walls of the refringent bodies. Fig. 7 shows 
some of the latter cut through at different levels, and here the 
rods are plainly seen in situ. The rods alone are represented 
in fig. 12. 
