424 WM. CECIL BOSANQUET. 
a more marked variety—an apparently more massive crystal— 
was seen. Washing out the benzol once more and then adding 
water caused the crystalline appearance to vanish almost 
entirely, a faint semblance of crossing lines in the substance 
of the granule being all that was left visible (fig. 3, 6. c. d.), 
while this substance itself first became opaque and then cleared 
again. The re-addition of the former. reagents caused the 
reappearance of the crystals. Oil of cloves similarly used 
seemed even more effectual in their production than benzol. 
What the nature of the phenomenon may be is difficult to say. 
It may be that true crystals are formed, modified by their 
organic origin and surroundings, being precipitated from 
alcohol and benzol and redissolved in water. On the other 
hand, it may be suggested that such an appearance could be 
produced by a splitting of the substance of the granule in 
consequence of a shrinkage caused by the reagents. This 
explanation seems perhaps to harmonise better with the 
phenomenon of the faint lines left on the disappearance of the 
crystal in water, i.e. a line of division remained to mark the 
original cleft ; also in many cases where the granules appeared 
split into two halves the line of cleavage corresponded very 
closely with the dark lines forming the limbs of the stars 
(fig. 3 g, A), which lines seemed in some instances to extend from 
edge to edge of the granule. Further, in one case at least I 
thought I could detect a forking of the end of one of the radiat- 
ing lines which would not be possible in a crystal, and in one 
instance when the objective was accidentally screwed down so 
as to touch the cover-glass and crush the section beneath, the 
flattening of the granules was accompanied by entire dis- 
appearance of the “ crystals.”” This explanation (viz. as hollows 
in the granules) is given by Wolters (8) of the rather different 
crystalline appearance seen by him in another gregarine, but 
the apparent staining of the “ crystals’ in some preparations 
seems a difficulty in the way of this explanation.! The same 
1 Actual crystals were found by Frenzel (4) in other Gregarines, but these 
appear to occur not in, but among the granules. Might not these crystals be 
comparable to the crystals found in plants, which apparently are excretory 
products ? 
