428 WM. CECIL BOSANQUET. — 
ing their spore-coat (fig. 14). The nucleus is placed almost 
invariably to one side, on the equator of the sporoblast. 
Spores and Falciform Bodies.—The formation of spores 
from the sporoblasts appears to begin at the outside of the 
cyst, and in one preparation the spores seemed to be so ar- 
ranged as to point outwards, with their long axis on the radii of 
the cyst (fig. 15), the centre being occupied by sporoblasto- 
meres. In many cases a number of granules remain unab- 
sorbed till the end. It may be noted that the characteristic 
knobs at the end of the spores seem to disappear entirely in 
permanent preparations, either dissolving in the reagents or 
becoming invisible owing to their possessing an index of re- 
fraction equal to that of Canada balsam. In glycerine these 
processes were still visible. In two cases out of a large number 
of cysts examined I have seen aspecimen of the curious tri- 
angular spores figured by Lieberkuhn, which are probably 
monstrosities. The development of the spores proceeds as 
follows :—The nucleus divides into two, then each fragment 
divides again, and the four resulting fragments divide once 
more. There are thus formed eight small round nuclei, lying 
in the protoplasm of the spore (fig. 16). The exact process of 
division, with or without mitosis, I have not been able to 
follow. The protoplasm surrounding these nuclear fragments 
divides, and a portion surrounds each, these portions elongating 
aud forming the falciform bodies. The nucleus in these lies 
almost at the extreme end (fig. 17). The arrangement of the falci- 
form bodies in the spore does not appear to be constant, the most 
favourite being perhaps that in which the nuclei or heads lie 
towards the centre, the tails pointing four to either end of the 
spore (fig. 17 6). The falciform bodies are coloured very prettily 
by the Ehrlich-Biondi stain; the nuclei appearing green, the 
body pink.! Their shape seems to vary considerably, some being 
longer, others thicker ; but it is possible that these differences 
represent stages in their elongation and development. 
1 In the mature Gregarines the nucleoli were stained a bright pink by this 
reagent, the ground-substance of the nucleus taking the same colour, but 
very faintly, 
