214 
plasm. The slide may then be warmed gently over a 
flame until the film begins to dry. It may then be 
stained by placing a drop of safranin or dahlia over the 
film. After a few minutes (three or four) the excess of 
stain may be removed by washing with a little 50 per 
cent. and 70 per cent. spirit. A few drops of absolute 
alcohol are then run over the film, followed by oil of 
cloves, and finally the preparation is mounted in balsam. 
(2) The film may be allowed to gradually dry (a 
matter of only a minute or two if it be even and thin), 
but before it is actually quite dry a little saturated 
solution of corrosive sublimate is gently run over it and 
the whole slide immersed for a few minutes in this 
solution. The slide is then washed in the usual way in 
water and in the alcohols (several hours), and may then 
be stained by any of the methods apphed to films or 
sections, é.g., ivon-alum-hematoxylin, hemalum, safranin, 
dahha, carmine. ‘These preparations show the various 
ceelomic cells and also the reproductive products. 
S€ C10 ms). 
Specimens not above six inches long are large enough 
for this purpose. Larger ones are more difficult to deal 
with on account of the hardness of the musculature after 
imbedding. 
Specimens intended for sectioning must, of course, be 
treated so as to remove all the sand from the alimentary 
canal, and it is a matter of some little difficulty to ensure 
this. The worms should be kept in dishes of clean sea 
water, into which a small stream of water is allowed to 
trickle or else the water in the dishes should be changed 
at least twice a day. The dishes should be examined 
several times a day, and the sand, which has been voided by 
the worms, removed. After four or five days of this treat- 
