PREPARATION OF EYE FOR HISTOLOGICAL EXAMINATION, 607 
The Preparation of the Eye for Histological 
Examination. 
By 
James W. Barrett, M.B., 
Demonstrator of Physiology in King’s College, London. 
Durine the last eighteen months I have been endeavouring 
to prepare satisfactory sections of different portions of the 
eye, and the following communication, which treats only of 
method, is based upon the results obtained. 
I am especially anxious that no statement in it shall be 
regarded as being final, since I feel convinced that success in 
this as in other branches of histology depends as much on 
the histologist’s knowledge of a method as on its intrinsic 
merits. My results may, however, serve to indicate the direc- 
tion in which success is to be sought. 
Tur PREPARATION OF SECTIONS OF THE EntTIRE Eye. 
I do not think that sections of an entire eye can be pre- 
pared without the aid of embedding and infiltrating materials, 
and I have been successful with only two, celloidin and 
paraffin. 
1. Preparation of sections of entire eyes by infiltrating and 
embedding in celloidin. 
The eye, removed from the body as soon as possible 
after death, should be opened by a short incision through the 
sclerotic, midway between the cornea and the entrance of the 
optic nerve, and should then be placed in some fixing and 
hardening agent (Miiller’s fluid, chromic acid solution). Ulti- 
