PREPARATION OF EYE FOR HISTOLOGIOAL EXAMINATION, 609 
according to the rapidity with which it stains ; the staining is 
somewhat diffuse, and it is sometimes preferable to place the 
eye after staining in alcohol containing a trace of HCl in 
order to remove the stain from everything but the nuclei. 
Formula for Alcoholic Borax Carmine (Woodward’s). 
Carmine, Nr. 40, gr. xv; 
Borax 3] ; 
Water to 8 oz. 
Dissolve by warming and slowly evaporate to 4 0z.; now add 7 oz. of 
alcohol. 
If it is to be used for staining in bulk there is no need to 
filter it. It should be shaken well from time to time. 
After the eye is stained it should be washed and transferred 
to alcohol, and then to a mixture of alcohol and ether equal 
parts. In this mixture the eye should be left for tweuty- 
four hours, when it should be transferred to a thin solution 
of celloidin in equal parts of absolute alcohol and ether ; an 
accurate measurement of the quantities of alcohol and ether 
is unnecessary, but the quantity of ether should never be 
greater than the quantity of alcohol. 
In this solution the eye may be left for two or three days 
until the celloidin has fairly penetrated all parts of it. 
Embedding.—The infiltrated eye should be placed in a 
pill-box or paper boat with a perfectly flat floor, and a 
tolerably thick solution of celloidin should be poured into the 
box until the eye is completely covered. The box or boat 
should then be placed on a glass plate and should be covered 
with a bell jar; the alcohol and ether diffuse into the air 
beneath the bell jar and the celloidin slowly consolidates. If 
a bell jar is not used a crust usually forms on the surface 
of the celloidin and further evaporation is hindered, whilst 
on the other hand the use of the bell jar permits of an 
equable removal of the alcohol and ether from all parts of 
the mass without the formation of bubbles. It should 
be lifted from time to time to allow of a partial removal of 
the gaseous alcohol and ether. The use of the bell jar is 
